Zonal Sedimentation Analysis on Sucrose Gradients.
نویسنده
چکیده
Zonal sedimentation analysis on sucrose gradients allows estimation of the molecular size of an individual protein or a protein complex by centrifugation at a constant speed under nondenaturing conditions. This method is particularly suitable for globular proteins like the influenza A virus (IAV) protein hemagglutinin (HA). Here, I describe step by step a protocol used to evaluate the oligomeric state of recombinant HA trimers (Magadan et al., 2013).
منابع مشابه
Nucleic acid of rubella virus and its replication in hamster kidney cells.
Ribonucleic acid (RNA) has been isolated from partially purified rubella virus preparations and fractionated by rate zonal centrifugation in sucrose density gradients. The bulk of the RNA sedimented as a sharp band with a sedimentation coefficient of 38S. Rubella virus RNA appears to be single-stranded on the basis of its sensitivity to the degrading action of ribonuclease. Fractionation by pre...
متن کاملDensity gradient centrifugation studies on rabies virus.
Neurath, A. Robert (The Wistar Institute, Philadelphia, Pa.), Tadeusz J. Wiktor, and Hilary Koprowski. Density gradient centrifugation studies on rabies virus. J. Bacteriol. 92:102-106. 1966.-Cesium chloride density gradient centrifugation of rabies virus revealed a heterogeneous population of infectious virus particles, the majority of which showed a density of 1.20 g/ml. From results obtained...
متن کاملIsolation of native glycogen by combined rate-zonal and isopycnic centrifugation.
Rat liver glycogen was separated into fractions of uniform particle size by the rate-zonal centrifugation of homogenates through cesium chloride and sucrose density gradients. Glycogen was then isolated from other cell constituents having similar sedimentation coefficients by isopycnic-zonal centrifugation in CsCI density gradients to effect a density separation. A density of 1.62 to 1.65 was i...
متن کاملPurification and electron microscopy of lactic dehydrogenase virus of mice.
Lactic dehydrogenase virus (LDV) was purified from infectious ascites fluid of mice bearing Ehrlich tumours using Sepharose gel filtration and rate zonal and isopycnic sedimentation. In glycerol gradients, a sedimentation coefficient of about 200S and a buoyant density of 1-14 g/ml was determined for the virus particle. Spherical particles with diam. between 62 and 80 nm, depending on the metho...
متن کاملHigh-throughput polyribosome fractionation.
Polyribosome sedimentation velocity centrifugation can be used to identify differential regulation of the translation of mRNAs. However, ultracentrifugation presents practical limitations on the number of sedimentation velocity gradients that can be run simultaneously. A method for sedimentation velocity analysis of polyribosomes is presented that is based on low-speed centrifugation of sucrose...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Bio-protocol
دوره 4 7 شماره
صفحات -
تاریخ انتشار 2014